Accelerating and Streamlining Microbial Process Development with a Novel Technology

Addressing key bottleneck in microbial process development with ShunzymeX™

Microbial expression systems enable rapid, cost-effective production of diverse therapeutics but often require bespoke downstream processes that extend timelines and increase risk. We developed ShunzymeX, a high purity, engineered protease designed to enable scarless removal of affinity tags and to standardize purification across a broad range of microbial proteins. ShunzymeX exhibits high specificity, solubility, and low impurity burden, allowing in-process use without creating clearance challenges. Proof-of-concept studies across four model proteins expressed in the antibiotic-free Paveway™ strain demonstrated >95% cleavage efficiency at low enzyme-to-substrate ratios, with robust capture by metal affinity chromatography and streamlined flow-through polishing. This platform accelerates and streamlines microbial workflows and addresses a key bottleneck in microbial bioprocessing, enabling predictable, scalable, and accelerated manufacturing of next-generation biologics. Download the white paper to learn more.